QIAcuity One 5-Plex Digital PCR System

The QIAcuity Digital PCR System is designed to deliver precise and multiplexed quantification results for mutation detection, copy number variation (CNV), gene expression studies, gene-editing analysis, and many more. This nanoplate-based system seamlessly integrates a standard dPCR workflow of partitioning, thermocycling and imaging into a walk-away automated platform with minimal hands-on time.

QIAcuity features and benefits:

  • Scalable design
  • The highest throughput digital PCR system ever
  • Ultra-high multiplexing
  • Fully automated digital PCR
  • Simplified transition from qPCR

To learn more about digital PCR, here is a brief Introduction to PCR and digital PCR and a presentation on the QIAcuity digital PCR assay optimization: Best practices and guidance.

PX1 PCR Plate Sealer

The PX1 PCR plate sealer provides consistent and uniform sealing across an entire microplate. This semiautomated heat sealer is ready to seal in less than 3 minutes. The PX1 sealer removes human variability from plate sealing and minimizes sample evaporation from the PCR workflow. This versatile instrument is compatible with a variety of heat-sealing films and foils and a wide range of PCR plates.

The PX1 PCR plate sealer features an intuitive touch-screen user interface. The thermal sealing process is simplified by allowing sealing temperature and time to be modified with the touch of a button.

Key Features and Benefits

  • Fast startup time — avoid delaying an experiment while the sealer warms up
  • Extremely intuitive — save time programming the instrument
  • Small footprint — accommodates crowded laboratory benches
  • Quickly access sealing protocols — save time by easily using stored protocols


OT-2 Robot

Need an open source programmable robot? The OT-2 from Opentrons is an easy-to-use pipetting robot that uses integrated electronic pipettes to transfer liquids and run automated moderate to high-throughout experiments in 96 well plates.

QX100 ddPCR

Bio-Rad’s QX100 ddPCR system provides an absolute measure of target DNA molecules with unrivaled precision and accuracy. The QX100 system partitions each sample into 20,000 individual nanoliter-sized droplets. PCR-positive and PCR-negative droplets from every sample are then counted to provide absolute target quantification in digital form. The QX100 system provides a revolutionary approach to target DNA quantification.

ABI Real-Time PCR System (384-well)

The Applied Biosystems® QuantStudio® 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio® 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 5 coupled channels and white LED), the QuantStudio® 5 384-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio® 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.

If you are looking for a recommendation on what types of plates to use with the machine, we suggest and typically use the MicroAmp Optical 384-Well Reaction Plate with Barcode.


The ABI PRISM 7900HT Sequence Detection System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5’ nuclease assay. The ABI PRISM 7900HT Sequence Detection System is equipped with a blue laser and can be configured to have either 96 or 384 well blocks.