Nucleic Acid Quantitation

NanoDrop

Measure the concentration and purity of your DNA, RNA or protein samples using the Thermo Scientific NanoDrop spectrophotometer and be confident in your downstream application results. Using only 1 µL, you can obtain your results in less than 15 seconds from sample pipetting to wiping the pedestal clean. > No dilutions required, even for highly concentrated samples >Patented sample retention system automatically optimizes pathlength to accommodate low and high concentrations (2 to 15,000 ng/µL dsDNA) >Only requires 0.5 – 2 µL of your sample >Calculates sample purity ratios (260/280 nm and 260/230 nm) >Pre-configured methods for DNA, Protein A280, Microarray, Protein and Labels, Pierce 660, Bradford, BCA, and Lowry >Fast measurement time of less than 5 seconds, 10 -15 seconds from sample pipette to wipe >User friendly software that includes custom methods and data export capabilities

Perkin Elmer Envision

The EnVision® 2105 multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Tried and tested, the EnVision microplate reader gives you robust performance and reliable data for high throughput screening, time after time.

The EnVision 2105 model delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP).

Technologies included

  • Filter based fluorescence intensity (top and bottom)
  • Time-resolved fluorescence (top and bottom)
  • Luminescence, including glow, flash and dual luminescence
  • Filter-based absorbance
  • Fluorescence Polarization
  • Dispensers

Fluorescence filters include 260/280, 405 nm, DAPI/Hoechst/BFP/Alexa350/AMC/Umbelliferone, mCherry/Alexa594/Texas Red, FITC,  BODIPY/TAMRA, and maybe additional ones that PCAT users purchase.

QX100 ddPCR

Bio-Rad’s QX100 ddPCR system provides an absolute measure of target DNA molecules with unrivaled precision and accuracy. The QX100 system partitions each sample into 20,000 individual nanoliter-sized droplets. PCR-positive and PCR-negative droplets from every sample are then counted to provide absolute target quantification in digital form. The QX100 system provides a revolutionary approach to target DNA quantification.

ABI Real-Time PCR System (384-well)

The Applied Biosystems® QuantStudio® 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio® 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 5 coupled channels and white LED), the QuantStudio® 5 384-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio® 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.

If you are looking for a recommendation on what types of plates to use with the machine, we suggest and typically use the MicroAmp Optical 384-Well Reaction Plate with Barcode.

Bioanalyzer

Agilent 2100 bioanalyzer is an analysis tool capable of handling nucleic acids, proteins and cells on one platform. When combined with an Agilent kit, this lab-on-a-chip technology can expedite conventional biochemical analysis. One of the benefits the Agilent 2100 bioanalyzer has over conventional bioanalytical methods is the elimination of time consuming procedures. Lab-on-a-Chip technology simplifies the process of data gathering and analysis down to three quick and easy steps: Load sample, run analysis, view data. The Agilent 2100 bioanalyzer utilizes micro-fabricated chips with up to 12-wells requiring minimum sample consumption, in the low μl-range. Prepackaged reagents included with the chip kits help to speed up the entire process.

7900HT QPCR

The ABI PRISM 7900HT Sequence Detection System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5’ nuclease assay. The ABI PRISM 7900HT Sequence Detection System is equipped with a blue laser and can be configured to have either 96 or 384 well blocks.