Data Analysis and Storage

Is your -80ºC on the fritz? Do you simply need to temporarily thaw your freezer to clean it out? The PCAT holds a spare and provides scheduling for a spare large -80ºC Freezer. If you are not a member of the DC, you should first confirm that you have checked with UCSF Facilities to see whether they have a loaner for you.

This model is a Thermo Scientific Revco UxF Ultra-Low Temperature Freezer, and holds ~28 cubic feet storage and it is located on the 10th floor, outside of the PCAT space. Ideally, it is not to be moved so bring your freezer racks on a cart to this location. If it absolutely does need to be moved, most likely facilities can do this for you.

Here are the FAQs:

• To use the freezer-- you must sign up for the duration of your intended use.
• This freezer is most frequently used as a temporary (2-3 days) loaner freezer for routine freeze-thaw clean outs of your existing freezer. 
• If your lab needs to occupy the freezer for longer than a few days, for example, while waiting for new freezer to be delivered, discuss your timeline with the PCAT. You should provide evidence that your replacement freezer has been ordered.
• This loaner is maintained in an unplugged state when not in use. Hence it will take approximately 12-24 hours to sufficiently cool down. It is a green policy. In most cases, your broken freezer will maintain a frozen state for a day or two, without dry ice, and without opening it.
• After use, and to prevent mould growth, the freezer must be left clean and dry.

Designed for biologists, Harmony’s workflow-based interface makes the whole process of high-content analysis straightforward, even for new users with little microscopy or programming knowledge.

One high-content analysis software for all your applications

• Phenotypic Screening
  • Get robust phenotypic fingerprints based on intensity, morphology, texture and fluorescence distribution parameters
  • Easily train the software to recognize relevant phenotypes
  • Quantify even subtle phenotypic differences
• Live Cell Imaging
  • Follow phenotypic changes over time
  • Accurately quantify label-free live cell images
  • Track individual cells over time or analyze wound-healing assays
  • Analyze cell signaling dynamics using e.g. FRET based assays
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  • Better understand your cell models by exploring them in the new 3D viewer and the XYZ viewer
  • Measure morphologies and volumes in 3D, count nuclei within spheroids
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• Rare Cell Phenotypes
  • Quickly capture your rare cell phenotypes with PreciScan^™
  • Pre-screen at low resolution and acquire objects of interest at high resolution
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• Routine Assays
  • Utilize more than 30 ready-made-solutions for standard assays
  • Ensure repeatability by running established protocols

One high-content analysis software for all users

• New Users
  • Get quantification for your cell images
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  • Get started with 30 ready-made solutions
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• Expert Users
  • Quantify difficult phenotypes with advanced texture and morphology readouts
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• For Lab Managers
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  • View and analyze data from any computer with installation of Harmony software
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