This microfluidics microscopy workstation, based on the original Drop-seq design, provides a highly versatile platform for innovative single-cell and microscale biological applications beyond traditional transcriptomics. Unlike commercial systems like 10X Chromium, this setup allows unparalleled flexibility for custom workflows, such as co-encapsulation of cells and reagents for cell-cell interaction studies, targeted single-cell functional assays, or encapsulating non-cellular materials like synthetic RNA or protein libraries. With integrated microscopy, researchers can visualize and monitor droplet generation or cellular processes in real time, enabling dynamic experiments such as live-cell imaging within droplets or the analysis of fluorescent reporters. The system is particularly suited for exploratory and proof-of-concept studies where commercial platforms lack customization or adaptability, providing an open framework for cutting-edge microfluidic technology development.